Detection of Newcastle disease virus using nucleic acid sequence-based amplification.

نویسندگان

  • Shang-Jin Cui
  • Yin-Wan Wendy Fung
  • Lok Ting Lau
  • Wen-Bin Liu
  • Yun-Feng Wang
  • Guang-Zhi Tong
  • Jianguo Chen
  • Albert Cheung Hoi Yu
چکیده

Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance.

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عنوان ژورنال:
  • Biologicals : journal of the International Association of Biological Standardization

دوره 35 1  شماره 

صفحات  -

تاریخ انتشار 2007